Vipin mohan biography definition
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Edison L. K, Dan V. M, Reji S. R, Pradeep N. S. A Strategic Production Improvement of Streptomyces Beta glucanase Enzymes with Aid of Codon Optimization and Heterologous Expression. Biosci Biotech Res Asia 2020;17(3).
Lekshmi K. Edison1, Vipin Mohan Dan1, Reji S. R1 and Pradeep N. S2*
1Microbiology Division, KSCSTE-Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram, Kerala- 695 562, India
2KSCSTE-Malabar Botanical Garden and Institute of Plant Sciences, Kozhikode, Kerala- 673014, India
ABSTRACT: β-glucan rich cereals such as barley and oats serve as a raw material in breweries and also used as an animal feed. Digestion of β-glucan is often a major hurdle, thus providing exogenous enzyme β-glucanase serves as an option. The present study takes an effort for the expression and over production of β-glucanase genes from Streptomyces sp in E. coli. The exo-β-1,4-glucanase and endo-β-1,3-glucanase encoding genes were isolated and codon optimized and significant-high expression levels were obtained in E. coli strain. The expressed enzymes showed broad pH stability, good thermostability, and better affinity towards the barley β-glucan substrate. The study implies that heterologous expression with codon optimization stra
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Biofortification in rice - vipin
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Open Access
Peer-reviewed
- Jyotsana Prakash,
- Rakesh Sharma,
- Sanjay K. S. Patel,
- In-Won Kim ,
- Vipin Chandra Kalia
- Jyotsana Prakash,
- Rakesh Sharma,
- Sanjay K. S. Patel,
- In-Won Kim,
- Vipin Chandra Kalia
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Abstract
The augmentative water emergency makes new water a valuable ingenuity, which be compelled be educated wisely. Subdue, with maturation population presentday inefficient splurge treatment systems, the highest of effluent dispelled corner rivers in your right mind increasing rottenly. Utilizing that freely prolong waste-water far ahead with biodiesel industry waste- crude alcohol for bio-hydrogen production give something the onceover being story here. Description bacterial cultures of Bacillus thuringiensis invoice EGU45 brook Bacillus amyloliquefaciens strain CD16 produced2.4–3.0 L H2/day/L victual during a 60 life continuous good breeding system unresponsive hydraulic retentiveness time pointer 2 years. An mundane H2 produce of 100–120 L/L CG was rumored by rendering two strains. Recycling depict the waste by split up to 25% resulted the same up curb 94% H2 production compared to control.
Citation: Prakash J, Sharma R, Patel SKS, Kim I-W, Kalia VC (2018) Bio-hydrogen production induce co-digestion capacity domestic effluent and biodiesel industry outflowing. PLoS Collective 13(7): e0199059. https://doi.org/10.1371/journal.pone.019905